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Extra resources for Alginic Acid: Chemical Structure, Uses and Health Benefits (Chemistry Research and Applications)
623. , S. Kasapis, and M. G. Gothard, Structural and textural properties of calcium induced, hot-made alginate gels. Carbohydr. , 1994. 24(3): p. 199–207. , Evaluation of a novel alginate gel dressing: cytotoxicity to fibroblasts in vitro and foreign-body reaction in pig skin in vivo. J. Biomed. Mater. , 1998. 39(2): p. 317-22. , Stress-relaxation behavior in gels with ionic and covalent crosslinks. J. Appl. , 2010. 107(6): p. 63509.
Alginate-based nanosphere preparation. g. sonication) can highly affect to the size of the particles. Additionally, internal or external gelation can also lead to different homogeneity [36, 52]. Some variations of this methodology have been recently developed, such as the use of a w/o/w emulsion  or a single step procedure . However, any of these emulsion based technologies is suitable for an industrial scale application in contrast with nozzle procedures, which make them ideal processes to obtain alginate based nanoparticles.
It has been also demonstrated that the use of multi-functional cross-linking molecules, such as poly(acrylamide-co-hydrazide) (PAH) or adipic acid dihydrazide (AAD), allows a better control over degradation rates and mechanical properties to be obtained than with bi-functional cross-linker molecules. Additionally, photo cross-linking reactions using appropriate chemical initiators can be also used to obtain covalent cross-linked alginate gels . c) Thermal Gelation Thermo-sensitive hydrogels have been widely used as drug release devices due to the possibility of adjusting their swelling ratio according to temperature conditions.